Glen or Glenda

Timothy Farrell Dr. Alton / Narrator

Bela Lugosi Scientist

Edward D. Wood Jr. Glen / Glenda (as Daniel Davis)

Lyle Talbot Inspector Warren

Dolores Fuller Barbara

'Tommy' Haynes Alan / Anne

Charlie Crafts Johnny (as Charles Crafts)

Conrad Brooks Banker / Reporter / Pickup Artist / Bearded Drag (as Connie Brooks)

Henry Bederski Man with Hat and Receding Hairline (uncredited)

Carol Daugherty Woman in Nightmare (uncredited)

'Captain' DeZita The Devil / Glen's Father (uncredited)

Helen Miles (uncredited)

Shirley Speril Miss Stevens (uncredited)

Amzie Strickland Minor Role (uncredited)

Harry Thomas Man in Nightmare (uncredited)

William C. Thompson Judge (uncredited)

'Mr. Walter' Hadjwiecyz Patrick / Patricia (uncredited)

George Weiss Man at Transvestite's Suicide (uncredited)

Evelyn Wood Sheila, Glen's Sister (uncredited)